Each technological challenge dictates the choice of a specific type of enzyme or, in most cases, a combination of enzymes.
Amylases, for example, are widely used when the goal is to improve the softness, volume, and color of bread. They belong to the hydrolase family and break down starch bonds through hydrolysis reactions.
However, efficient starch conversion does not depend on a single enzyme. This process involves the coordinated action of different types of amylases, such as:
- Fungal and bacterial alpha-amylases
- Beta-amylases
- Maltogenic amylases
- Pululanases
- Amyloglucosidases
- Alpha-glucosidases
In addition, there are enzymes that do not break down starch but rather rearrange its structure, such as cyclodextrin glycosyltransferase, further expanding technological possibilities.
Given so many options within the same enzyme family, an inappropriate selection can make product development longer, more complex, and more costly. Therefore, an efficient experimental plan must begin with a deep understanding of each enzyme’s mechanism of action and its direct correlation with the challenge to be solved.