Six multicatheterized dairy cows in their second lactation were used to investigate the effects of increased glucose absorption from the small intestine on the supply of glucose and amino acids to peripheral tissues. The cows were randomly assigned to treatments Control or Infusion of 1,500 g glucose/d into the abomasum from the day of calving to 29 days in milk. Blood samples were obtained from an artery, the portal vein and the liver vein at 14 day before expected calving as well as 4, 15 and 29 days in milk. Dry matter intake (DMI) and milk yield was lower with glucose infusion, whereas the milk composition was not affected. Absorption of glucose from the small intestine to portal blood was increased by glucose infusion, but the release of glucose from the liver was decreased, due to the lower dry matter intake. Consequently, the supply of glucose to peripheral tissues was not affected in terms of g/d, but was numerically increased in terms of g pr. kg DMI. The net absorption of total and essential amino acids from the small intestine to the portal blood tended to be decreased with glucose infusion, due to the decreased DMI. In contrast, was the release of amino acids from the liver not affected by the glucose infusion. Thus, was the supply of total and essential amino acids to the peripheral tissues lower with glucose infusion in terms of g/d, whereas it was not affected in terms of g pr. kg DMI. In conclusion, increased absorption of glucose from the small intestine increased the amount of glucose available for milk production and thus increased the glucogenic status. In contrast, was the amount of amino acids available for milk production not affected by increased glucose absorption from the small intestine. The present results indicate that feeding bypass starch to dairy cows does not enhance the protein utilization by reducing the intestinal and liver use of amino acids.